NAD+

Mechanism

Universal cofactor supporting ATP production and sirtuin activity.

Reported effects

What sources associate with this compound. Reported categories, not outcomes we have graded — each would need its own citation and rung.

• Cellular energy
• DNA-repair support
• Mitochondrial function

Half-life, storage & sport

Elimination half-life

not on file — no primary pharmacokinetic study found.

Storage

Reconstituted: In aqueous buffer: highly stable in Tris at 19C (>90% remaining at 43 days) and 25C (75% at 43 days); faster degradation in phosphate or HEPES (up to 34 uM/day); NAD+ degrades base-catalysed at high pH, optimum ~pH 8.5. — source. Molecules 2024 long-term cofactor stability study; no pharmaceutical vial shelf-life on file.

In sport (WADA 2026)

Not prohibited. Not named. Separate trap: method M2.2 prohibits intravenous infusions of more than 100 mL per 12 hours, which catches many NAD+ IV drips regardless of the substance. the list

No half-life is on file for this compound, and the figures circulating in its place do not agree with each other. 4 separate accounts are recorded on this page, read from 5 pages between them: approximately 45 minutes, 1 to 5 minutes, 4 to 10 minutes and 48 to 72 hours. They run from 1 min to 3 d, a factor of 4,320. No curve is drawn below, because drawing one would mean choosing a winner among them and publishing that choice as though something had measured it. Read what each one says, and where it was read from.

Literature on file 5

• review or editorial
  The role of redox state in regulating plant secondary metabolism under stress. graded on: indexed as "Review" - secondary literature, not a study — “Review”
• review or editorial
  The Role of Oxidative Stress in Heart Failure: Mechanisms and Therapeutic Targets. graded on: indexed as "Review" - secondary literature, not a study — “Review”
• review or editorial
  Nicotinamide adenine dinucleotide phosphate oxidase 4 in lung disease: a review of its biology and therapeutic potential. graded on: indexed as "Review" - secondary literature, not a study — “Review”
• animal in vivo
  Nicotinamide Adenine Dinucleotide as a Potential Biomarker for Graft Viability During Normothermic Machine Perfusion-An Ex Vivo Rodent Study. graded on: an animal model — “Rodent”
• computational
  Autophagy-Related Gene-Set Signals in the Genetic Architecture of Obsessive-Compulsive Disorder: A Hypothesis-Generating Link to Nicotinamide Adenine Dinucleotide-Associated Cellular Maintenance Biology. graded on: TWAS-derived gene-level statistics tested for KEGG pathway enrichment; no wet-lab endpoint — read from the abstract — “abstract review”

What is circulated not evidence 4

Claims passed around in community guides and vendor copy, recorded because they circulate — not because they are true. Each is shown with the page it was read from and what this record actually holds. Checked 2026-08-26.

• unverified community data half life common nothing on file either way

  A plasma half-life of about 45 minutes is stated for injected NAD+, assigned to the subcutaneous route on one page and to the intravenous route on another.

  Exogenous NAD+ administered subcutaneously has an estimated plasma half-life of approximately 45 minutes.

  On the record No half-life for NAD+ is on file, by any route. This figure is the most repeated of the four that circulate, and no page stating it names a study, a species, an assay or a number of subjects. The two pages that carry it also disagree about which route it describes.

  Source 1 · Source 2

• unverified community data half life occasional nothing on file either way

  A plasma half-life of one to five minutes after intravenous NAD+ is stated and attributed to Trammell et al., Nature Communications 2016.

  plasma half-life of approximately 1–5 minutes

  On the record No half-life for NAD+ is on file. The paper this figure cites is a single-dose oral nicotinamide riboside trial: it administers no NAD+ intravenously and reports no NAD+ half-life. Trammell does report a 2.7-hour half-life, and it belongs to nicotinamide riboside taken by mouth, which is a different molecule by a different route. Search summaries now repeat this figure with the citation attached, which is what makes it look sourced.

  Source 1

• unverified community data half life occasional nothing on file either way

  A half-life of four to ten minutes is stated for intravenous NAD+, with subcutaneous described as longer and given no figure.

  Approximately 4–10 minutes (IV); longer for subcutaneous.

  On the record No half-life for NAD+ is on file. This figure carries no citation anywhere it appears, and it disagrees with the 45-minute figure on the pages beside it by roughly an order of magnitude.

  Source 1

• unverified community data half life fringe nothing on file either way

  Forty-eight to seventy-two hours is given in the half-life position, describing how long the effects of an infusion are said to persist.

  The half-life of NAD+ is IV: effects persist 48-72 hours (2-3 days)

  On the record No half-life for NAD+ is on file. This is not a half-life at all: the sentence states how long an effect is reported to last, printed under a heading that asks for the elimination of the molecule. The two are different quantities and this record does not hold either.

  Source 1

Identity

PubChem CID 5892 computed 3D conformer

Skeletal formulathe canonical depiction

Drawn by PubChem from CID 5892, the identifier on this record.

Molecular weight

663.4

Molecular formula

C21H27N7O14P2

CAS number

53-84-9

PubChem CID

5892

InChI key

BAWFJGJZGIEFAR-NNYOXOHSSA-N

SMILES

C1=CC(=C[N+](=C1)[C@H]2[C@@H]([C@@H]([C@H](O2)COP(=O)([O-])OP(=O)(O)OC[C@@H]3[C@H]([C@H]([C@@H](O3)N4C=NC5=C(N=CN=C54)N)O)O)O)O)C(=O)N

InChI

InChI=1S/C21H27N7O14P2/c22-17-12-19(25-7-24-17)28(8-26-12)21-16(32)14(30)11(41-21)6-39-44(36,37)42-43(34,35)38-5-10-13(29)15(31)20(40-10)27-3-1-2-9(4-27)18(23)33/h1-4,7-8,10-11,13-16,20-21,29-32H,5-6H2,(H5-,22,23,24,25,33,34,35,36,37)/t10-,11-,13-,14-,15-,16-,20-,21-/m1/s1

Classified as

Mitochondrial Functionpathway

The phrase appears in a great deal of product copy but is measured very differently across studies, from oxygen consumption in cultured cells to exercise capacity in a person, and the rung a claim earns usually depends on which was actually done.

Also called

• NAD+

Listed prices 394

What 212 shops we track listed for NAD+, read from their own public catalogues on 2026-09-14. We take no commission on these and they are not ranked by any payment — how vendors are scored.

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